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Our content explains pharmacopoeial and regulatory requirements in our own words, for learning. For exact requirements, always refer to the current official text and your site's approved SOPs. More

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Sectors / Molecular / Core Techniques

Core Techniques

Extraction, PCR, electrophoresis and ELISA.

Courses

Certificate courses from beginner to advanced.

PREMIUMintermediateCore Techniques

Molecular Biology Laboratory Techniques

Good molecular practice, DNA and RNA extraction, PCR optimisation, qPCR and ΔΔCt analysis, cloning basics, ELISA and protein methods.

7 lessons · 6 h · ₹1,499

Virtual practicals

Simulated practicals with new data every attempt and a certificate for each one you pass. Results are simulated for learning.

Agarose Gel Electrophoresis

Cast a gel of the right strength, load samples and a ladder, run towards the correct electrode and size your bands.

Calculators

Each tool shows the formula, a worked example and its limitations.

DNA/RNA concentration from A260Concentration from absorbance at 260 nm.FREE DNA copy numberCopies of a dsDNA template from its mass and length.FREE qPCR efficiency from the standard curve slopeAmplification efficiency from a Ct vs log quantity slope.PRO Primer melting temperature (basic)Quick Tm estimate for a primer sequence.FREE

FREE10 min

DNA Quantity and Purity

Read A260 and A280, calculate concentration, judge purity and dilute DNA to a working concentration.

FREE12 min

Setting Up a PCR Master Mix

Scale a PCR recipe to the number of reactions, calculate volumes from stock concentrations and choose the right controls.

PRO12 min

qPCR Data Analysis: Efficiency and ΔΔCt

Check efficiency from a standard curve, calculate ΔCt and ΔΔCt and report fold change.