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Microbiologist.in
Pharma QC microbiology, in your pocket
Note
Concentration = 0.5 × 50 µg/mL × 10 = 250 µg/mL (ng/µL). Reliable mainly for A260 between about 0.1 and 1.0.
Reference: A260 of 1.0 ≈ 50 µg/mL dsDNA, 33 µg/mL ssDNA, 40 µg/mL RNA (1 cm path). Always follow your approved SOP and current pharmacopoeia.
Concentration = A260 × factor × dilution
A260 0.5, dsDNA, 1 in 10 → 250 µg/mL
Proteins, phenol and free nucleotides affect A260; check A260/A280 and A260/A230.