DNA copy number

FREE

Copies of a dsDNA template from its mass and length.

Note

Copies = (1 ng × 10⁻⁹ × 6.022 × 10²³) / (3,000 bp × 650 g/mol) = 3.09 × 10^8 copies.

Reference: Avogadro's number and 650 g/mol per base pair. Always follow your approved SOP and current pharmacopoeia.

Formula

Copies = (ng × 10⁻⁹ × 6.022 × 10²³) / (bp × 650)

1 ng of a 3000 bp plasmid → 3.09 × 10⁸ copies

Some sources use 660 g/mol per bp; accuracy depends on the concentration measurement.