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Microbiologist.in
Pharma QC microbiology, in your pocket
Manual WBC, RBC, platelet or fluid cell counts from the Neubauer chamber.
Note
Count = 120 × 20 / (4 mm² × 0.1 mm) = 6,000 cells/µL.
Reference: Cells/µL = cells counted × dilution / (area in mm² × depth in mm). For learning only. Reference intervals vary by laboratory, method and patient group; results are interpreted by qualified professionals with the clinical picture.
Cells/µL = cells counted × dilution factor / (area counted in mm² × depth 0.1 mm)
120 WBC in the 4 corner squares (4 mm²) at 1:20 → 120 × 20 / 0.4 = 6,000 /µL
Uneven filling, clumping and too few cells counted increase error; count both sides of the chamber where your SOP requires.
For learning only. Reference intervals vary by laboratory, method and patient group; results are interpreted by qualified professionals with the clinical picture.