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Biochemical oxygen demand from dissolved oxygen readings.
Pro
BOD₅ = (D₁ − D₂) / P, P = sample volume / bottle volume
DO 8.6 → 4.2 mg/L, 10 mL in a 300 mL bottle → 132 mg/L
Seeded samples need a seed correction; acceptable dilutions deplete at least 2 mg/L DO and leave at least 1 mg/L.