Molecular Diagnostics Essentials

What you'll be able to do

• ✓ Handle samples for DNA and RNA testing
• ✓ Extract nucleic acids and check quality
• ✓ Explain PCR and design pitfalls
• ✓ Read real-time PCR curves and Ct values
• ✓ Run gel electrophoresis and understand sequencing basics
• ✓ Organise a molecular lab to prevent contamination
• ✓ Apply controls, verification and EQA

Syllabus

1. MODULE 1Samples and extractionNucleic acids, samples and extraction.

   • Nucleic acids and samples for molecular testingFree preview
   • Nucleic acid extractionFree preview
2. MODULE 2Amplification and detectionPCR, real-time PCR and electrophoresis.

   • PCR: how amplification works
   • Real-time PCR and Ct values
   • Electrophoresis and sequencing basics
3. MODULE 3The molecular laboratoryDesign, contamination control and quality.

   • Laboratory design and contamination control
   • Quality and validation in molecular diagnostics
   • Final exam (20 questions)

Questions