MVD (maximum valid dilution)

The maximum dilution of a product at which the endotoxin limit can still be detected, calculated as (endotoxin limit × concentration of the test solution) ÷ λ. Diluting a product reduces interference with the BET, but diluting beyond the MVD would mean endotoxin at the limit could no longer be detected. A more sensitive lysate, with a lower λ, gives a larger MVD and more room to overcome interference.